Translational_Unit

Part:BBa_K902051:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)

dszA behind and RBS (B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 700
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1084


Design Notes

See DszA mutations


Source

Amplified from an environmental isolate of R. erythropolis. This part was constructed using the basic biobrick assembly method.

References

Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003.